Robertson L W, Parkinson A, Chittim B, Bandiera S, Sawyer T W, Safe S
Toxicology. 1981;22(2):103-14. doi: 10.1016/0300-483x(81)90110-4.
Irradiation of the commercial polybrominated biphenyl (PBB( mixture, fireMaster BP-6, in cyclohexane solution at 300 nm for 930 min resulted in a marked diminution of the major components of the mixture. Administration of the photolyzed PBB mixture of fireMaster BP-6 to immature male Wistar rats caused both dose-related decreases in thymus weight and increase in hepatic microsomal benzo[a]pyrene hydroxylase (AHH), 4-dimethylaminoantipyrine N-demethylase and NADPH-cytochrome c reductase activities and cytochrome P-450 content. The dose effecting half-maximal AHH induction for the photolyzed PBBs (9 mg . KG-1) was approximately 6 times lower than that of fireMaster BP-6 (50 mg. kg-1). Furthermore, the concentration of photolyzed PBBs (2 micrometers) required to displace 50% of the specifically-bound [3H] TCDD from its high-affinity cytosolic Ah receptor was approximately 150 times lower than that required for fireMaster BP-6 (300 micrometers), as measured by sucrose density gradient centrifugation analysis. The results suggest that the photolysis of the commercial PBB mixture yields products which possess increased biologic activity.
在300纳米波长下，对商业多溴联苯（PBB）混合物FireMaster BP - 6的环己烷溶液进行930分钟的辐照，导致该混合物的主要成分显著减少。将经光解的FireMaster BP - 6的PBB混合物给予未成熟雄性Wistar大鼠，会导致胸腺重量呈剂量相关下降，同时肝微粒体苯并[a]芘羟化酶（AHH）、4 - 二甲基氨基安替比林N - 脱甲基酶和NADPH - 细胞色素c还原酶活性以及细胞色素P - 450含量增加。光解后的PBB诱导AHH达到半数最大效应的剂量（9毫克·千克⁻¹）约为FireMaster BP - 6（50毫克·千克⁻¹）的六分之一。此外，通过蔗糖密度梯度离心分析测得，将50%特异性结合的[³H]TCDD从其高亲和力胞质Ah受体上置换下来所需的光解PBB浓度（2微摩尔）约为FireMaster BP - 6所需浓度（300微摩尔）的150分之一。结果表明，商业PBB混合物的光解产生了具有增强生物活性的产物。
