紫外线照射大鼠皮肤中速甾醇3的分离与鉴定。

Isolation and identification of tachysterol3 in rat skin exposed to ultraviolet light.

作者信息

Takada K, Kobayashi T

出版信息

Biochim Biophys Acta. 1981 Dec 23;666(3):356-60. doi: 10.1016/0005-2760(81)90294-0.

Abstract

In order to investigate the mechanism of the cutaneous synthesis of vitamin D3 in vivo, a photolysis by-product of previtamin D3 in organic solvent, namely, tachysterol3 was isolated and identified in ultraviolet-exposed rat skin. Tachysterol3 was identified by chromatographic comigration of an ultraviolet-absorbing peak wih authentic tachysterol3 in straight-phase HPLC (Zorbax SIL column) and reversed-phase HPLC (Nucleosil 5C18 column), and by the ultraviolet absorption spectrum. The comparison of the extent of formation of tachysterol3 between in rat skin in vivo and in ethanol solution indicated that the yield in rat skin is similar to that in organic solvent. The results suggest that the reaction mechanism in rat skin in vivo may be identical wit that in organic solvents.

摘要

为了研究体内皮肤合成维生素D3的机制，在紫外线照射的大鼠皮肤中分离并鉴定了前维生素D3在有机溶剂中的光解副产物，即速甾醇3。通过在正相高效液相色谱（Zorbax SIL柱）和反相高效液相色谱（Nucleosil 5C18柱）中，将一个紫外线吸收峰与纯速甾醇3进行色谱共迁移，以及通过紫外线吸收光谱，鉴定了速甾醇3。体内大鼠皮肤与乙醇溶液中速甾醇3形成程度的比较表明，大鼠皮肤中的产率与有机溶剂中的产率相似。结果表明，体内大鼠皮肤中的反应机制可能与有机溶剂中的相同。