Wren S F, Salerno T A, Crowe M, de Bold A J
Can J Surg. 1981 Nov;24(6):604-5.
Through a study of the activity of the regulatory enzyme of glycolysis phosphofructokinase and the regulatory enzyme of gluconeogenesis fructose-1,6-diphosphatase, myocardial metabolism has been evaluated in the human heart during cardiopulmonary bypass. Three ventricular transmural biopsy specimens were obtained at open-heart surgery before cardiopulmonary bypass was instituted and three similar specimens were taken 30 minutes after cardiopulmonary bypass was started. The enzyme assays were performed on the supernatant obtained from myocardial cells disrupted by sonication. The activity of both enzymes increased during the anoxic conditions of cardiopulmonary arrest. The increase in phosphofructokinase activity is likely due to an overall increase in glycolytic activity, but the presence and increased activity of fructose-1,6-diphosphatase was unexpected.
通过对糖酵解的调节酶磷酸果糖激酶和糖异生的调节酶果糖-1,6-二磷酸酶的活性进行研究，对体外循环期间人体心脏的心肌代谢进行了评估。在体外循环开始前的心脏直视手术中获取了三个心室透壁活检标本，并在体外循环开始30分钟后获取了三个类似的标本。酶测定是在通过超声处理破坏心肌细胞后获得的上清液上进行的。在心肺骤停的缺氧条件下，两种酶的活性均增加。磷酸果糖激酶活性的增加可能是由于糖酵解活性的总体增加，但果糖-1,6-二磷酸酶的存在及其活性增加是出乎意料的。
