Groothuis G M, Hulstaert C E, Kalicharan D, Hardonk M J
Eur J Cell Biol. 1981 Dec;26(1):43-51.
It was investigated whether rat hepatocytes maintain their plasma membrane specialization (sinusoidal, lateral and bile canalicular sites) and their intracellular polarity (peribiliary region, rich in lysosomes and poor in mitochondria) after isolation. The morphology of the hepatocytes and the cytochemical localization of marker enzymes for the bile canalicular membrane (alkaline phosphatase, adenosine triphosphatase and 5' nucleotidase), for the lysosomes (acid phosphatase) and for the mitochondria (beta-hydroxybutyrate dehydrogenase and succinate dehydrogenase) were studied in situ and directly after isolation using both light and electron microscopy. The morphology of the cells and the cytochemical activity of acid phosphatase, succinate dehydrogenase and beta-hydroxybutyrate dehydrogenase showed that in isolated cells, as in situ, the lysosomes were concentrated in bands, devoid of mitochondria. Unlike in situ the reaction product of alkaline phosphatase, adenosine triphosphatase and 5'nucleotidase was evenly distributed along the entire plasma membrane of the isolated cells. Morphologically, no tight or gap junctions or desmosomes could be detected in the isolated cells, while the plasma membrane appeared to be homogeneously covered with uniform microvilli. In conclusion it can be stated that during isolation the hepatocytes loose their distinct plasma membrane specialization, but maintain their peribiliary region rich in lysosomes and poor in mitochondria.
研究了大鼠肝细胞在分离后是否能维持其质膜特化（窦状、侧面和胆小管部位）及其细胞内极性（胆小管周围区域，富含溶酶体而线粒体较少）。使用光学显微镜和电子显微镜，在原位以及分离后直接对肝细胞的形态以及胆小管膜标记酶（碱性磷酸酶、三磷酸腺苷酶和5'核苷酸酶）、溶酶体（酸性磷酸酶）和线粒体（β-羟基丁酸脱氢酶和琥珀酸脱氢酶）的细胞化学定位进行了研究。细胞形态以及酸性磷酸酶、琥珀酸脱氢酶和β-羟基丁酸脱氢酶的细胞化学活性表明，与原位情况一样，分离后的细胞中溶酶体集中成带，且不含线粒体。与原位不同的是，碱性磷酸酶、三磷酸腺苷酶和5'核苷酸酶的反应产物在分离细胞的整个质膜上均匀分布。在形态上，分离后的细胞中未检测到紧密连接、间隙连接或桥粒，而质膜似乎均匀地覆盖着均匀的微绒毛。总之，可以说在分离过程中，肝细胞失去了其明显的质膜特化，但维持了富含溶酶体而线粒体较少的胆小管周围区域。
