Engelmann F
Mol Cell Endocrinol. 1981 Oct;24(1):103-12. doi: 10.1016/0303-7207(81)90082-4.
Fat bodies of adult vitellogenic or non-vitellogenic females and those of adult males contain 2 populations of saturable JH binding compounds. The respective Kd vales are 2-5 x 10(-9) M and 1-4 x 10(-8) M. Fat bodies of penultimate nymphs, hemolymph of any animal, and midgut epithelia contain one saturable JH binder with a Kd of 1-5 x 10(-8) M. Unlabeled JH I and III, as well as the JH analog ZR-515 compete effectively for the binding sites in these tissues. The 2 classes of hormone binders were separated by DEAE ion-exchange column chromatography. The very high affinity molecules of the adult fat bodies are presumably the specific JH receptors which may function in translocation of the hormone to the nucleus. The compound with relatively lower hormone affinity may be the JH hemolymph carrier which is also produced by the fat bodies and then exported.
成年卵黄生成期或非卵黄生成期雌性以及成年雄性的脂肪体中含有两类可饱和的保幼激素结合化合物。各自的解离常数（Kd）值分别为2 - 5×10⁻⁹M和1 - 4×10⁻⁸M。倒数第二龄若虫的脂肪体、任何动物的血淋巴以及中肠上皮含有一种可饱和的保幼激素结合剂，其Kd为1 - 5×10⁻⁸M。未标记的保幼激素I和III以及保幼激素类似物ZR - 515能有效竞争这些组织中的结合位点。这两类激素结合剂通过二乙氨基乙基（DEAE）离子交换柱色谱法分离。成年脂肪体中具有极高亲和力的分子可能是特异性的保幼激素受体，其可能在将激素转运至细胞核的过程中发挥作用。激素亲和力相对较低的化合物可能是保幼激素血淋巴载体，它也是由脂肪体产生然后输出的。
