Engelmann F
Department of Biology, University of California, Los Angeles 90024, USA.
Insect Biochem Mol Biol. 1995 Jun;25(6):721-6. doi: 10.1016/0965-1748(95)00011-j.
The juvenile hormone (JH) receptor has been isolated and purified from fat bodies of females and males of Leucophaea maderae. The sequence of procedures that yielded an apparently pure compound involved ammonium sulfate precipitation (20-60% saturation cut), DEAE anion exchange column chromatography, and polyacrylamide gel electrophoresis. The molecular size of the resulting high affinity JH-binding protein is about 64 kDa and is apparently composed of two equal subunits of 32 kDa. A polyclonal antibody has been produced against this molecule and used for the construction of an immunoaffinity column. A pure JH receptor molecule has been isolated from crude fat body homogenates with this affinity column. The JH receptor is first identified in last instar nymphs and quantitatively increases as the instar progresses towards adult development. In penultimate nymphal instars this JH receptor could not be identified and this is correlated with a lack of competence to make vitellogenin (Vg) upon exposure to the JH analog methoprene. Thus, a correlation is seen between the presence of the JH receptor and the competence to synthesize Vg.
已从地中海粉螟雌雄成虫的脂肪体中分离并纯化出保幼激素（JH）受体。获得一种看似纯净化合物的程序包括硫酸铵沉淀（20%-60%饱和度切割）、DEAE阴离子交换柱色谱法和聚丙烯酰胺凝胶电泳。所得高亲和力JH结合蛋白的分子大小约为64 kDa，显然由两个32 kDa的相等亚基组成。已针对该分子制备了多克隆抗体，并用于构建免疫亲和柱。利用该亲和柱已从粗脂肪体匀浆中分离出一种纯净的JH受体分子。JH受体首先在末龄若虫中被鉴定出来，并随着若虫向成虫发育而定量增加。在倒数第二龄若虫中无法鉴定出这种JH受体，这与接触JH类似物烯虫酯后缺乏合成卵黄原蛋白（Vg）的能力相关。因此，可观察到JH受体的存在与合成Vg的能力之间存在相关性。
