Issel C J, Adams W V, Meek L, Ochoa R
J Am Vet Med Assoc. 1982 Feb 1;180(3):272-5.
Twenty seven adult horses positive to the agar gel immunodiffusion (AGID) test for equine infectious anemia (EIA), but with no history of clinical EIA, were used in transfusion studies to determine whether infectious EIA virus was present in 1 to 5 ml of their blood. Of 27 recipients, 21 (78%) became AGID test-positive at an average of 24 days after inoculation. Two horses that were initially negative when screened were retested and found to carry infectious virus in 5-300 ml of whole blood; the other 4 horses were not retested. Horse flies (Tabanus fuscicostatus Hine) were unable to transmit EIA virus from 10 AGID test-positive donors with no history of clinical EIA, but virus was transmitted from a pony with artificially induced acute EIA and from a horse that had recovered from a clinical attack of EIA 9 months earlier. Histopathologic changes indicative of EIA were noted in all test-positive recipients. The most consistent lesion was paracortical lymphoid hyperplasia in the splenic lymph node.
27匹成年马琼脂凝胶免疫扩散试验（AGID）检测马传染性贫血（EIA）呈阳性，但无临床EIA病史，用于输血研究，以确定其1至5毫升血液中是否存在传染性EIA病毒。在27只接受输血的马中，21只（78%）在接种后平均24天AGID检测呈阳性。最初筛查时为阴性的两匹马重新检测后，发现其5至300毫升全血中携带传染性病毒；另外4匹马未重新检测。厩螫蝇（Tabanus fuscicostatus Hine）无法从10只无临床EIA病史的AGID检测呈阳性的供体传播EIA病毒，但病毒可从小马人工诱发的急性EIA以及9个月前从EIA临床发作中恢复的一匹马传播。在所有检测呈阳性的接受输血的马中均观察到指示EIA的组织病理学变化。最一致的病变是脾淋巴结副皮质区淋巴组织增生。
