De Larco J E, Preston Y A, Todaro G J
J Cell Physiol. 1981 Oct;109(1):143-52. doi: 10.1002/jcp.1041090116.
Serum-free conditioned media was collected from three sarcoma virus-transformed cell lines and an untransformed cell line. All three virally transformed lines produced and released growth factors into their serum-free media. The major activity in all cases, whether the cells were transformed by Moloney sarcoma virus (MSV) or Kirsten sarcoma virus (KiSV), or whether they were mouse or rat, was a sarcoma-growth-factor (SGF)-like activity with an apparent molecular weight of 10,000. The SGF-like pools from a Moloney sarcoma virus-transformed mouse 3T3 cell and a Kirsten sarcoma virus-transformed NRK cell were further purified by carboxymethyl cellulose chromatography. The elution profiles of these peptides were very similar. The serum-free conditioned media from the untransformed cells showed no detectable growth stimulating activity. The temperature sensitivity of an SGF-like growth factor from the supernate of a NRK cell transformed by a wild-type Kirsten sarcoma virus (KiSV) was compared with that of the SGF-like activity from the supernates of a NRK cell transformed by a ts-mutant of KiSV that is temperature sensitive with respect to transformation (ts-371 Cl 5). Neither the cells transformed by the wild-type sarcoma virus nor those transformed by the temperature sensitive virus released a SGF-like activity that was temperature sensitive under the conditions of the assays.
从三种肉瘤病毒转化的细胞系和一种未转化的细胞系中收集无血清条件培养基。所有三种病毒转化的细胞系都产生并向其无血清培养基中释放生长因子。在所有情况下，无论细胞是由莫洛尼肉瘤病毒（MSV）还是 Kirsten 肉瘤病毒（KiSV）转化，也无论它们是小鼠还是大鼠来源，主要活性都是一种表观分子量为 10,000 的肉瘤生长因子（SGF）样活性。来自莫洛尼肉瘤病毒转化的小鼠 3T3 细胞和 Kirsten 肉瘤病毒转化的 NRK 细胞的 SGF 样组分通过羧甲基纤维素色谱进一步纯化。这些肽的洗脱图谱非常相似。未转化细胞的无血清条件培养基未显示出可检测到的生长刺激活性。将野生型 Kirsten 肉瘤病毒（KiSV）转化的 NRK 细胞上清液中的 SGF 样生长因子的温度敏感性与 KiSV 的温度敏感突变体（ts - 371 Cl 5）转化的 NRK 细胞上清液中的 SGF 样活性的温度敏感性进行比较。在测定条件下，无论是野生型肉瘤病毒转化的细胞还是温度敏感病毒转化的细胞，都未释放出温度敏感的 SGF 样活性。
