以Percoll作为分离介质，通过单位重力下的速度沉降对淋巴细胞进行快速大规模分级分离。
Rapid large scale fractionation of lymphoid cells by velocity sedimentation at unit gravity, using Percoll as separation medium.
作者信息
Opstelten D, Deenen G J, Bos L, Hermse A, Muurman L, Nieuwenhuis P
出版信息
J Immunol Methods. 1982;48(2):189-98. doi: 10.1016/0022-1759(82)90193-4.
Available methods for separation of cells by velocity sedimentation at unit gravity allow a maximum cell load of 2 x 108 lymphoid cells. Here we describe a method, based on the 'tilting' procedure of Bont et al. (1979), which allows separation of up to 4.5 x 109 lymphoid cells, while retaining full separation and rapidity. Percoll instead of Ficoll was used as gradient medium. The relationship between cell sedimentation velocity and cell volume was analyzed by two different methods, i.e., electronic cell sizing and diameter measurements of cytocentrifuged cells.
通过单位重力下的速度沉降分离细胞的现有方法允许的最大细胞负载量为2×10⁸个淋巴细胞。在此，我们描述一种基于Bont等人（1979年）的“倾斜”程序的方法，该方法可分离多达4.5×10⁹个淋巴细胞，同时保持完全分离和快速性。使用Percoll代替Ficoll作为梯度介质。通过两种不同方法分析细胞沉降速度与细胞体积之间的关系，即电子细胞大小测定和细胞离心涂片的直径测量。
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