Kang Y H, West W L
J Morphol. 1982 Jan;171(1):1-10. doi: 10.1002/jmor.1051710102.
The effect of ovarian hormones on the activities of glucose-6-phosphatase and alkaline phosphatase in the vaginal epithelium was studied in immature and ovariectomized rats, using ultracytochemical techniques. Comparative studies were done on normal rats at the luteal phase and on day 14 of pregnancy. Various vaginal cells show different degrees of response to progesterone and diethylstilbestrol (DES) with regard to glucose-6-phosphatase activity. Intense glucose-6-phosphatase activity was observed in the cisternae of granular endoplasmic reticulum (rER), Golgi saccules and vesicles, and nuclear envelope of both basal cells and stromal cells of progesterone treated rats, whereas in the basal cells and stromal cells of DES-treated and control animals the enzyme was totally lacking. Detectable glucose-6-phosphatase activity was also observed, however, in the rER cisternae and Golgi complex of keratohyalin-secreting squamous intermediate cells of the vaginal epithelium of DES-treated rats. Alkaline phosphatase was also found on the limiting membranes of secretory granules of mucocytes in animals at the luteal phase and during pregnancy. DES and progesterone in the doses used did not affect alkaline phosphatase activity in the rat vagina. Overall, progesterone enhances glucose-6-phosphatase activity in basal cells of the rat vagina prior to completion of mucification. Alkaline phosphatase was found in all cells involved in mucin secretion.
采用超微细胞化学技术，在未成熟和去卵巢大鼠中研究了卵巢激素对阴道上皮中葡萄糖-6-磷酸酶和碱性磷酸酶活性的影响。对处于黄体期的正常大鼠和妊娠第14天的大鼠进行了比较研究。就葡萄糖-6-磷酸酶活性而言，各种阴道细胞对孕酮和己烯雌酚（DES）表现出不同程度的反应。在孕酮处理大鼠的基底细胞和基质细胞的颗粒内质网（rER）池、高尔基体囊泡和小泡以及核膜中观察到强烈的葡萄糖-6-磷酸酶活性，而在DES处理动物和对照动物的基底细胞和基质细胞中该酶完全缺失。然而，在DES处理大鼠阴道上皮分泌透明角质的鳞状中间细胞的rER池和高尔基体复合体中也观察到了可检测到的葡萄糖-6-磷酸酶活性。在黄体期和妊娠期动物的黏液细胞分泌颗粒的限制膜上也发现了碱性磷酸酶。所用剂量的DES和孕酮不影响大鼠阴道中的碱性磷酸酶活性。总体而言，在黏液化完成之前，孕酮增强了大鼠阴道基底细胞中的葡萄糖-6-磷酸酶活性。在所有参与黏蛋白分泌的细胞中都发现了碱性磷酸酶。
