Rayment I, Baker T S, Caspar D L, Murakami W T
Nature. 1982 Jan 14;295(5845):110-5. doi: 10.1038/295110a0.
X-ray diffraction data from polyoma capsid crystals were phased by refinement of low-resolution starting models to obtain a self-consistent structural solution. The unexpected result that the hexavalent morphological unit is a pentamer shows that specificity of bonding is not conserved among the protein subunits in the icosahedrally symmetric capsid.
来自多瘤病毒衣壳晶体的X射线衍射数据通过对低分辨率起始模型进行精修来确定相位,以获得自洽的结构解决方案。六价形态单元是五聚体这一意外结果表明,在二十面体对称衣壳中的蛋白质亚基之间,键合特异性并不保守。