Douglas S D
J Immunol. 1978 Jan;120(1):151-7.
Freeze fracture studies have been performed on rabbit pulmonary alveolar macrophages and a nonphagocytic murine lymphoblastoid cell line, PU-5 Fc+, incubated with sheep erythrocytes, sheep erythrocyte-IgG Forssman antibody complex, sheep erythrocyte-IgG Forssman antibody-C complexes and aggregated IgG. Alveolar macrophages show redistribution of intramembrane particles after interaction with (EIgG) and E(IgM)C. The murine lymphoblastoid cell line shows intramembrane particle redistribution consequential to binding of E(IgG) and aggregated IgG. The results demonstrate that after specific immunoprotein receptor-ligand interaction, there is extensive plasma membrane reorganization which results in a redistribution and loss of intramembrane particles. Changes are observed in the protoplasmic face of the plasma membrane after the binding of ligand to the outer membrane surface. The findings suggest that interaction of erthrocyte-bound ligands with specific lymphoid and macrophage plasma membrane receptors leads to a generalized redistribution of integral membrane components in the membrane.
已对兔肺泡巨噬细胞和一种非吞噬性小鼠淋巴母细胞系PU - 5 Fc +进行了冷冻断裂研究，该细胞系与绵羊红细胞、绵羊红细胞 - IgG福斯曼抗体复合物、绵羊红细胞 - IgG福斯曼抗体 - C复合物以及聚集的IgG一起孵育。肺泡巨噬细胞在与（EIgG）和E（IgM）C相互作用后显示膜内颗粒重新分布。小鼠淋巴母细胞系显示出因E（IgG）和聚集的IgG结合而导致的膜内颗粒重新分布。结果表明，在特异性免疫蛋白受体 - 配体相互作用后，会发生广泛的质膜重组，导致膜内颗粒重新分布和丢失。在配体与外膜表面结合后，在质膜的原生质面观察到变化。这些发现表明，红细胞结合的配体与特定淋巴细胞和巨噬细胞质膜受体的相互作用导致膜中整合膜成分的普遍重新分布。
