Speth V
Acta Histochem Suppl. 1981;23:173-80.
Freeze-fracturing and deep-etching have been used to characterize the internal morphology and surface charge of the plasma membrane of GH3-cells from cell culture in fixed and unfixed material. Glutaraldehyde does not effect intramembrane particle pattern but induces structural modifications on the cell surface in a way that additional attachment sites for cationized ferritin are exposed at specific areas of the membrane. Negative surface charges are not directly linked to the intramembrane particles, but to other (surface-) proteins, independently enough to be dislocated by external ligands; this process is restricted to the membrane surface. Thermotropic phase changes induce separation of lipidic regions from proteinaceous regions. Peripheral and integral protein are thus aggregated simultaneously, resulting in clustered membrane particles and aggregated surface charges.
冷冻断裂和深度蚀刻已被用于表征来自细胞培养的GH3细胞在固定和未固定材料中的质膜内部形态和表面电荷。戊二醛不影响膜内颗粒模式，但会以一种方式诱导细胞表面的结构修饰，即在膜的特定区域暴露阳离子化铁蛋白的额外附着位点。负表面电荷不直接与膜内颗粒相连，而是与其他（表面）蛋白质相连，其独立性足以被外部配体移位；这个过程仅限于膜表面。热致相变诱导脂质区域与蛋白质区域分离。因此，外周蛋白和整合蛋白同时聚集，导致膜颗粒聚集和表面电荷聚集。
