Lobo R A, Goebelsmann U, Brenner P F, Mishell D R
Am J Obstet Gynecol. 1982 Feb 15;142(4):471-8. doi: 10.1016/s0002-9378(16)32393-6.
The serum levels of adrenal androgens (aa) are lower in oophorectomized (OO) than in ovulating (OV) women. This study was carried out in an effort to further investigate these findings and to study the effects of administration of estrogen on the levels of aa in OO women. Ten OO and seven OV women participated in this study in which aa were measured basally and after stimulation with adrenocorticotropic hormone (ACTH), both before and 4 weeks after conjugated estrogens (CE). Seven women received 0.625 mg of CE, and five received 2.5 mg of CE. Compared to OV women, OO women had significantly lower levels of androstenedione (Adione), dehydroepiandrosterone (DHEA) and its sulfate (DHEA-S), testosterone (T), delta 5-androstenediol (Adiol), and 17 beta-estradiol (E2) (p less than 0.01). In response to ACTH, OO women had smaller responses to Adione (p less than 0.05), DHEA (p less than 0.005), DHEA-S (p less than 0.01), 17-OH progesterone (17 Prog) (p less than 0.01), and 17-OH pregnenolone (17 Preg) (p less than 0.1). Furthermore, after ACTH, the ratio of 17 Prog/Adione was significantly higher in OO women (p less than 0.01), thus suggesting reduced 17,20-demolase (17,20D?) activity. Similarly, OO women had higher ratios of 127 Preg/17 Prog (p less than 0.1), DHEA/Adione (p less than 0.01), and Adiol/T (p less than 0.01), thereby suggesting reduced 3 beta ol dehydrogenase-isomerase (3 beta ol) activity. In response to CE, there was a dose-related increase in aa and cortisol. After 2.5 mg of CE, aa were significantly higher and similar to those levels in OV women. despite the known increases in sex hormone binding globulin-finding capacity and transcortin after estrogen, unbound T increased slightly, as did urinary free cortisol in women treated with 2.5 mg of CE. After treatment with estrogen, there was a dose-related change in the ACTH-stimulated steroid ratios that indicated and increase in 17,20D and 2 beta ol activities. In women who were gien 2.5 mg of CE, these enzyme activities were similar to those in OV women.
卵巢切除（OO）女性的血清肾上腺雄激素（aa）水平低于排卵（OV）女性。本研究旨在进一步探究这些发现，并研究雌激素给药对OO女性aa水平的影响。10名OO女性和7名OV女性参与了本研究，在服用结合雌激素（CE）之前和之后4周，分别在基础状态和促肾上腺皮质激素（ACTH）刺激后测量aa。7名女性接受0.625mg的CE，5名接受2.5mg的CE。与OV女性相比，OO女性的雄烯二酮（Adione）、脱氢表雄酮（DHEA）及其硫酸盐（DHEA-S）、睾酮（T）、δ5-雄烯二醇（Adiol）和17β-雌二醇（E2）水平显著更低（p<0.01）。对ACTH的反应中，OO女性对Adione（p<0.05）、DHEA（p<0.005）、DHEA-S（p<0.01）、17-羟孕酮（17Prog）（p<0.01）和17-羟孕烯醇酮（17Preg）（p<0.1）的反应较小。此外，ACTH刺激后，OO女性的17Prog/Adione比值显著更高（p<0.01），这表明17,20-裂解酶（17,20D？）活性降低。同样，OO女性的127Preg/17Prog（p<0.1）、DHEA/Adione（p<0.01）和Adiol/T（p<0.01）比值更高，从而表明3β-羟类固醇脱氢酶-异构酶（3βol）活性降低。对CE的反应中，aa和皮质醇呈剂量相关增加。服用2.5mg CE后，aa显著更高且与OV女性的水平相似。尽管已知雌激素后性激素结合球蛋白结合能力和皮质激素转运蛋白增加，但未结合的T略有增加，服用2.5mg CE的女性尿游离皮质醇也是如此。雌激素治疗后，ACTH刺激的类固醇比值发生剂量相关变化，表明17,20D和2βol活性增加。在服用2.5mg CE的女性中，这些酶活性与OV女性相似。
