Crum C P, Braun L A, Shah K V, Fu Y S, Levine R U, Fenoglio C M, Richart R M, Townsend D E
Cancer. 1982 Feb 1;49(3):468-71. doi: 10.1002/1097-0142(19820201)49:3<468::aid-cncr2820490313>3.0.co;2-7.
Immunoperoxidase localization of a human papilloma virus structural antigen (HPV) was attempted in 68 intraepithelial lesions of the vulva, 39 of which were analyzed for nuclear DNA content by microspectrophotometry. Overall, 5.9% (4/68) stained positive for HPV. Ninety percent (35/39) of the cases tested were aneuploid, and, of these, 2.8% (1/35) stained positive for HPV. In contrast, 50% (2/4) of the polyploid lesions were positive. Hence DNA microspectrophotometry and immunoperoxidase localization of HPV are useful coparameters for distinguishing wart virus infection (condylomata) from vulvar intraepithelial neoplasia. HPV is detected infrequently within aneuploid lesions, in keeping with the concept that epithelial maturation is required for virion assembly. Whether the HPV genome exists in a nonreplicative state within the aneuploid cell population is unknown.
对68例外阴上皮内病变进行了人乳头瘤病毒结构抗原（HPV）的免疫过氧化物酶定位，其中39例通过显微分光光度法分析了核DNA含量。总体而言，5.9%（4/68）的HPV染色呈阳性。检测的病例中有90%（35/39）为非整倍体，其中2.8%（1/35）的HPV染色呈阳性。相比之下，50%（2/4）的多倍体病变呈阳性。因此，DNA显微分光光度法和HPV免疫过氧化物酶定位是区分疣病毒感染（尖锐湿疣）和外阴上皮内瘤变的有用共同参数。在非整倍体病变中很少检测到HPV，这与病毒体组装需要上皮成熟的概念一致。非整倍体细胞群内HPV基因组是否以非复制状态存在尚不清楚。
