Gupta J, Pilotti S, Rilke F, Shah K
Am J Pathol. 1987 May;127(2):206-15.
The authors examined paraffin sections from 85 genital tract tissues from 49 cases for the presence of human papillomavirus (HPV) Types 6/11, 16, and 18 by stringent in situ hybridization using 35S-labeled viral DNA probes, and for viral capsid antigen by the immunoperoxidase test. The cases, selected mostly on the basis of vulvar pathology, were distributed as follows: early neoplasia (Group I, 6 cases); early neoplasia with viral cytopathic effect (CE) (Group II, 24 cases); and papillomavirus infection (PVI) (Group III, 19 cases). Available tissues from all affected sites were examined when the disease was multicentric. One or more viral DNAs were identified in 58% of 77 tissues from Groups II and III and in 2 of 8 tissues from Group I. HPV-6/11, HPV-16 and HPV-18 DNAs were detected, respectively, in 25, 24, and 2 tissues; 3 tissues were infected simultaneously with either two or three viruses. Viral DNA was identified at more than one site in 14 of 30 DNA-positive patients; in 10 of these, a single type was detected at all sites in the same patient. The viral DNA was localized mostly in areas showing viral cytopathology. The presence of HPV-16 correlated with neoplasia. HPV-16 DNA was identified in the 2 virus-positive tissues showing neoplasia, in 17 of 20 (85%) of the DNA-positive tissues showing neoplasia with CE, and in 5 of 25 (20%) of the DNA-positive tissues showing PVI. Conversely, HPV-6/11 was found in 25% of the DNA-positive tissues showing neoplasia with CE and in 80% of the cases of PVI. An HPV genome was identified in neoplastic cells in 14 instances; in all but 1 case, the genome was HPV-16. The association of HPV-16 with neoplasia was seen for both vulvar and cervical lesions. Viral antigen was detected in 83% of lesions associated with HPV 6/11 and in 62% of lesions associated with HPV-16.
作者使用35S标记的病毒DNA探针，通过严格的原位杂交检查了49例患者85份生殖道组织石蜡切片中是否存在人乳头瘤病毒（HPV）6/11型、16型和18型，并通过免疫过氧化物酶试验检测病毒衣壳抗原。这些病例大多根据外阴病理学进行选择，分布如下：早期肿瘤形成（I组，6例）；有病毒细胞病变效应（CE）的早期肿瘤形成（II组，24例）；以及乳头瘤病毒感染（PVI）（III组，19例）。当疾病为多中心性时，对所有受累部位的可用组织进行检查。在II组和III组的77份组织中有58%以及I组的8份组织中有2份检测到一种或多种病毒DNA。分别在25份、24份和2份组织中检测到HPV-6/11、HPV-16和HPV-18 DNA；3份组织同时感染了两种或三种病毒。在30例DNA阳性患者中有14例在多个部位检测到病毒DNA；其中10例在同一患者的所有部位检测到单一类型的病毒。病毒DNA大多定位于显示病毒细胞病理学的区域。HPV-16的存在与肿瘤形成相关。在2份显示肿瘤形成的病毒阳性组织、20份（85%）显示有CE的肿瘤形成的DNA阳性组织中的17份以及25份（20%）显示PVI的DNA阳性组织中的5份中检测到HPV-16 DNA。相反，在25%显示有CE的肿瘤形成的DNA阳性组织以及80%的PVI病例中发现了HPV-6/11。在14例肿瘤细胞中鉴定出HPV基因组；除1例病例外，所有病例的基因组均为HPV-16。HPV-16与肿瘤形成的关联在外阴和宫颈病变中均可见。在与HPV 6/11相关的病变中有83%检测到病毒抗原，在与HPV-16相关的病变中有62%检测到病毒抗原。
