Kondor-Koch C, Garoff H
J Gen Virol. 1982 Feb;58(Pt 2):443-8. doi: 10.1099/0022-1317-58-2-443.
We have constructed a hybrid plasmid molecule that contains the complete coding sequences from the 26S mRNA of Semliki Forest virus. Five fragments which together covered the mRNA sequence were isolated from three original hybrid plasmids and joined together at five different restriction endonuclease cleavage sites using T4 DNA ligase.
我们构建了一种杂种质粒分子,它包含来自Semliki森林病毒26S mRNA的完整编码序列。从三个原始杂种质粒中分离出五个共同覆盖mRNA序列的片段,并使用T4 DNA连接酶在五个不同的限制性内切酶切割位点将它们连接在一起。