Frischauf A M, Garoff H, Lehrach H
Nucleic Acids Res. 1980 Dec 11;8(23):5541-9. doi: 10.1093/nar/8.23.5541.
We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are are generated. These can be sequenced directly without further strand- or fragment separation steps.
我们在此描述一种用于测序程序的片段制备新策略，该策略使用末端标记的DNA片段作为底物(2,3)，它可直接应用于克隆到pBR322的Pst I位点的DNA片段，或者以修饰形式应用于插入同一质粒的BamH I或Sal I位点的片段。生成具有预定重叠的有序亚克隆集。这些亚克隆无需进一步的链或片段分离步骤即可直接测序。
