Lodmell D L, Ewalt L C, Notkins A L
J Immunol. 1976 Nov;117(5 Pt.2):1757-61.
Induction of a celayed-type hypersensitivity reaction in tuberculin-sensitized animals by tuberculin purified protein derivative (PPD) at the site of dermal vaccinia virus (VV) infection markedly accelerated elimination of VV and led to clinical recovery. Viral titers were depressed by over 99.9% in the skin of animals given PPD as compared to animals given phosphate-buffered saline or nonspecific irritants. Low concentrations of acid labile interferon were found in the skin of uninfected tuberculin-sensitized animals challenged with PPD. High concentrations of acid stable interferon were found in skin of tuberculin-sensitized animals infected with VV and challenged with either PPD or PBS. The time of appearance of the acid stable interferon was markedly accelerated, however, in the animals challenged with PPD as compared with PBS. It is concluded that recovery from dermal vaccinia infection can be enhanced by induction of a local delayed-type hypersensitivity reaction with an antigen untelated to the infecting virus.
在皮肤牛痘病毒（VV）感染部位，通过结核菌素纯化蛋白衍生物（PPD）在结核菌素致敏动物中诱导迟发型超敏反应，可显著加速VV的清除并导致临床康复。与给予磷酸盐缓冲盐水或非特异性刺激物的动物相比，给予PPD的动物皮肤中的病毒滴度降低了99.9%以上。在用PPD攻击的未感染结核菌素致敏动物的皮肤中发现了低浓度的酸不稳定干扰素。在感染VV并用PPD或PBS攻击的结核菌素致敏动物的皮肤中发现了高浓度的酸稳定干扰素。然而，与PBS相比，在用PPD攻击的动物中，酸稳定干扰素出现的时间明显加快。结论是，通过用与感染病毒无关的抗原诱导局部迟发型超敏反应，可以增强皮肤牛痘感染后的恢复。
