Miyamae T
Avian Dis. 1981 Oct-Dec;25(4):1065-9.
Eighteen 2-year-old hens were infected orally with wild avian encephalomyelitis virus and examined using the immunofluorescent-antibody technique for 30 days. Viral antigen was detected in 10 organ tissues (liver, spleen, kidney, pancreas, and ovary) in 7 birds during the first half of the observation period and sporadically in alimentary tracts of 12 birds throughout this period, especially the lower parts of the intestines, but it was not detected in other organ tissues. Thirteen test-positive hens showed more localization of the antigen than baby chicks of other studies did.
18只2岁母鸡经口感染野生禽脑脊髓炎病毒，并运用免疫荧光抗体技术进行了30天的检测。在观察期的前半段，7只鸡的10种器官组织（肝脏、脾脏、肾脏、胰腺和卵巢）中检测到病毒抗原，在此期间，12只鸡的消化道中偶尔检测到病毒抗原，尤其是肠道下段，但在其他器官组织中未检测到病毒抗原。13只检测呈阳性的母鸡体内抗原的定位比其他研究中的雏鸡更多。
