Vazquez-Ramos J M, Mandelstam J
J Gen Microbiol. 1981 Nov;127(1):11-7. doi: 10.1099/00221287-127-1-11.
The effects of oxolinic acid and novobiocin - which respectively inhibit the A and B subunits of DNA gyrase, and therefore inhibit DNA synthesis - have examined in sporulating cultures of Bacillus subtilis. Although both inhibitors prevent sporulation, this is not due to inhibition of DNA synthesis. Instead, they affect protein synthesis generally, though weakly, but have very marked effects on the formation of individual enzymes. These effects are reproducible, but the type of enzyme that will be affected is not predictable. The results point to an involvement of DNA gyrase in the transcription of some genes. This is suggested as the reason for the effect of the inhibitors on spore formation, which they block mainly at Stage O-I.
已在枯草芽孢杆菌的芽孢形成培养物中研究了恶喹酸和新生霉素的作用，这两种物质分别抑制DNA促旋酶的A亚基和B亚基，从而抑制DNA合成。尽管两种抑制剂都能阻止芽孢形成，但这并非由于抑制了DNA合成。相反，它们通常会对蛋白质合成产生影响，尽管这种影响较弱，但对个别酶的形成有非常显著的作用。这些作用是可重复的，但受影响的酶的类型无法预测。结果表明DNA促旋酶参与了某些基因的转录。这被认为是抑制剂对孢子形成产生影响的原因，它们主要在O-I阶段阻断孢子形成。
