对具有不同生长史的猿猴肉瘤相关病毒蛋白质的研究。

Studies on Proteins of simian sarcoma-associated virus with different growth history.

作者信息

Warnaar S O, Huiskens-Van der Meij C, Kool-Kofoed L, Mooren H W

出版信息

J Gen Virol. 1982 Mar;59(Pt 1):65-71. doi: 10.1099/0022-1317-59-1-65.

Abstract

Simian sarcoma-associated virus (SSaV) was repeatedly passaged on three human cell lines. The proteins of the progeny virus were analysed for the presence of variant polypeptides. Occasionally, a few variant polypeptides were observed. One-dimensional peptide maps of the major virus protein p30 revealed no modifications after 25 cycles of infection on the three cell lines studied. The peptide map of Pr65gag of virus grown through 25 passages on a human chondrosarcoma cell line was slightly different from that of the virus stock before passaging. The relative amount of the virus protein p30 as compared to p18 and p16 (possibly the SSaV equivalents of p15E and p12E) was variable depending on the host cell. Virus grown on Daudi cells was relatively deficient in p18 and p16. These virus particles were morphologically altered and had a low infectivity.

摘要

猿猴肉瘤相关病毒（SSaV）在三种人类细胞系上反复传代。分析子代病毒的蛋白质中是否存在变异多肽。偶尔会观察到一些变异多肽。在所研究的三种细胞系上经过25轮感染后，主要病毒蛋白p30的一维肽图未显示出修饰。在人软骨肉瘤细胞系上经过25代传代培养的病毒的Pr65gag肽图与传代前的病毒原液略有不同。与p18和p16（可能相当于SSaV的p15E和p12E）相比，病毒蛋白p30的相对含量因宿主细胞而异。在Daudi细胞上生长的病毒相对缺乏p18和p16。这些病毒颗粒在形态上发生了改变，且感染性较低。