Herbrink P, van Bussel F J, Warnaar S O
Anticancer Res. 1982 Sep-Oct;2(5):291-6.
Antibodies cross-reactive with type C viral p30 were concentrated from 105 samples of human plasma by affinity chromatography using Sepharose beads to which disrupted Simian sarcoma associated virus (SSAV) had been coupled. Eluates obtained from the affinity beads were tested for anti-p30 activity by performing radio-immunoprecipitations with 125I-labeled SSAV p30 and Rauscher murine leukemia viral (R-MuLV) p30. Forty six percent of the eluates were found positive when tested against SSAV p30. From 44 positive eluates for anti SSAV p30 activity, 20 (45%) were also found to be positive for anti-R-MuLV p30 activity. No significant difference was found in anti-p30 activity in eluates from normal controls and from patients with various kinds of malignancy.
使用偶联了被破坏的猿猴肉瘤相关病毒（SSAV）的琼脂糖珠，通过亲和层析从105份人血浆样本中富集与C型病毒p30交叉反应的抗体。通过用125I标记的SSAV p30和劳斯氏鼠白血病病毒（R-MuLV）p30进行放射免疫沉淀，检测从亲和珠获得的洗脱液的抗p30活性。当针对SSAV p30进行检测时，发现46%的洗脱液呈阳性。在44份抗SSAV p30活性呈阳性的洗脱液中，20份（45%）抗R-MuLV p30活性也呈阳性。在正常对照者和各种恶性肿瘤患者的洗脱液中，抗p30活性未发现显著差异。
