Davis T P, Schoemaker H, Chen A, Yamamura H I
Life Sci. 1982 Mar 22;30(12):971-87. doi: 10.1016/0024-3205(82)90515-x.
Precise and quantitative reversed-phase high performance liquid chromatographic (HPLC) procedures are described which can be used in biogenic amine and neuropeptide research. The amine procedure was applied to various pharmacological matrices including plasma, heart tissue and brain. The use of peptide HPLC as an analytical tool for various neuropeptides is illustrated by studies on des-tyrosine-gamma-endorphin (DT gamma E) metabolism in the brain and the stability of an ACTH (ORG-2766) analogue during a chronic infusion in rats. The power of HPLC as a research tool in peptide pharmacology is described, discussed and demonstrated as an aid in the understanding of the pharmacological effects of exogenous peptides and the function of the brain.
本文描述了精确且定量的反相高效液相色谱（HPLC）方法，这些方法可用于生物胺和神经肽研究。胺类分析方法已应用于各种药理基质，包括血浆、心脏组织和大脑。通过对脑内去酪氨酸-γ-内啡肽（DTγE）代谢的研究以及促肾上腺皮质激素（ORG-2766）类似物在大鼠慢性输注过程中的稳定性研究，展示了肽HPLC作为各种神经肽分析工具的应用。文中描述、讨论并展示了HPLC作为肽类药理学研究工具的作用，以辅助理解外源性肽的药理作用和大脑功能。
