反相高效液相色谱法在一些关键肽分离中的应用。
Application of reversed-phase HPLC in some critical peptide separations.
作者信息
Janssen P S, van Nispen J W, Hamelinck R L, Melgers P A, Goverde B C
出版信息
J Chromatogr Sci. 1984 Jun;22(6):234-8. doi: 10.1093/chromsci/22.6.234.
PMID:6086687
Abstract
The application of reversed-phase high performance liquid chromatography in peptide chemistry is illustrated. Its use is described for separation of closely related endorphins, as an optical purity criterion, for rapid determination of the species of origin of insulin preparations, and finally for analysis of small, basic (hydrophilic) peptides.
摘要
本文阐述了反相高效液相色谱法在肽化学中的应用。介绍了其用于分离密切相关的内啡肽、作为光学纯度标准、快速测定胰岛素制剂的来源种类，以及最终用于分析小的碱性（亲水性）肽的用途。
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