Stohl L L, Collins R A, Cole M D, Lambowitz A M
Nucleic Acids Res. 1982 Mar 11;10(5):1439-58. doi: 10.1093/nar/10.5.1439.
Mitochondria from two Neurospora intermedia strains (P4O5-Labelle and Fiji N6-6) were found to contain plasmid DNAs in addition to the standard mitochondrial DNA species. The plasmid DNAs consist of monomeric circles (4.1-4.3 kbp and 5.2-5.3 kbp for Labelle and Fiji, respectively) and oligomers in which monomers are organized as head-to-tail repeats. DNA-DNA hybridization experiments showed that the plasmids have no substantial sequence homology to mtDNA, to each other, or to a previously characterized mitochondrial plasmid from N. crassa strain Mauriceville-lc (Collins et al. Cell 24, 443-452, 1981). The intramitochondrial location of the plasmids was established by cell fractionation and nuclease protection experiments. In sexual crosses, the plasmids showed strict maternal inheritance, the same as Neurospora mitochondrial DNA. The plasmids may represent a novel class of mitochondrial genetic elements.
研究发现，两种间型脉孢菌菌株（P4O5-Labelle和斐济N6-6）的线粒体除了含有标准的线粒体DNA种类外，还含有质粒DNA。质粒DNA由单体环（Labelle菌株和斐济菌株分别为4.1 - 4.3千碱基对和5.2 - 5.3千碱基对）和寡聚物组成，其中单体以头对尾重复的形式排列。DNA - DNA杂交实验表明，这些质粒与线粒体DNA、彼此之间或与之前鉴定的粗糙脉孢菌菌株Mauriceville - lc的线粒体质粒（Collins等人，《细胞》24卷，443 - 452页，1981年）没有实质性的序列同源性。通过细胞分级分离和核酸酶保护实验确定了质粒在线粒体内的位置。在有性杂交中，这些质粒表现出严格的母系遗传，与脉孢菌线粒体DNA相同。这些质粒可能代表了一类新型的线粒体遗传元件。
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