Collins R A, Stohl L L, Cole M D, Lambowitz A M
Cell. 1981 May;24(2):443-52. doi: 10.1016/0092-8674(81)90335-4.
We have identified a plasmid DNA that is found within mitochondria of wild-type N. crassa strain Mauriceville-1c (FGSC #2225), but that shows no detectable sequence homology with mitochondrial DNA. The plasmid DNA consists of an oligomeric series of circular molecules of monomer length 3.6 kb. There are two unusual clusters of restriction enzyme sites, one consisting of six Eco RI sites in a 1 kb region, and the other of five or more Pst I sites in a 0.4 kb region. RNA gel transfer hybridization experiments show a major transcript 3.3 to 3.4 kb long, close to the monomer length of the plasmid. The latter finding implies that the plasmid DNA contains specific sites for the initiation and termination of transcription.
我们已经鉴定出一种质粒DNA，它存在于野生型粗糙脉孢菌菌株莫里斯维尔-1c（FGSC #2225）的线粒体中，但与线粒体DNA没有可检测到的序列同源性。该质粒DNA由单体长度为3.6 kb的环状分子的寡聚系列组成。有两个不寻常的限制性酶切位点簇，一个在1 kb区域内由六个Eco RI位点组成，另一个在0.4 kb区域内由五个或更多Pst I位点组成。RNA凝胶转移杂交实验显示一条主要转录本，长度为3.3至3.4 kb，接近质粒的单体长度。后一发现表明质粒DNA含有转录起始和终止的特定位点。
