Eckfeldt J H, Light R T, Leiendecker-Foster C
Clin Chem. 1982 May;28(5):1108-12.
alpha 1-Antitrypsin is the most abundant of several serum protease inhibitors. Its deficiency is associated with an increased incidence of emphysema in adults, jaundice in newborns, and childhood cirrhosis. We describe an automated functional assay for the Instrumentation Laboratory's Multistat III Microcentrifugal Analyzer with N-alpha-benzoyl-DL-arginine-p-nitroanilide as trypsin substrate. The assay is standardized in terms of moles of trypsin active sites inhibited per liter of serum, by use of a chromogenic titrant for trypsin active sites, p-nitrophenyl-p'-guanidinobenzoate. The method is rapid, precise, and independent of trypsin supplier, and results correlate well with those by a manual chromogenic and a nephelometric assay.
α1-抗胰蛋白酶是几种血清蛋白酶抑制剂中含量最丰富的一种。其缺乏与成人肺气肿、新生儿黄疸和儿童肝硬化的发病率增加有关。我们描述了一种用于仪器实验室的Multistat III微量离心分析仪的自动化功能测定法，该方法以N-α-苯甲酰-DL-精氨酸对硝基苯胺作为胰蛋白酶底物。通过使用用于胰蛋白酶活性位点的显色滴定剂对硝基苯基-p'-胍基苯甲酸酯，该测定法以每升血清中抑制的胰蛋白酶活性位点的摩尔数进行标准化。该方法快速、精确，且与胰蛋白酶供应商无关，其结果与手动比色法和比浊法的结果相关性良好。
