Konings C H
Clin Chem. 1977 Sep;23(9):1760-3.
The Selected Method [Clin. Chem. 20, 396 (1974)] for the enzymatic assay of alpha1-antitrypsin in serum has been adapted for use with the E.N.I.-GEMSAEC. With alpha-N-benzoyl-D,L-arginine-p-nitroanilide as substrate, the difference between the tryptic activity measured with and without addition of serum in the same run has been used to calculate the trypsin-inhibitory capacity. The rate of increase in absorbance at 400 nm of the p-nitroanilide formed, has been evaluated during a reaction time of 140 s. Results correlated well (r = 0.986) for 54 human sera analyzed as described here and by the Selected Method. The adaptation on GEMSAEC can be used in detecting alpha1-antitrypsin deficiency in the newborn.
血清中α1-抗胰蛋白酶酶法测定的选定方法[《临床化学》20, 396 (1974)]已适用于E.N.I.-GEMSAEC。以α-N-苯甲酰-D,L-精氨酸对硝基苯胺为底物，在同一次测定中，加入血清和不加入血清时所测胰蛋白酶活性的差值用于计算胰蛋白酶抑制能力。在140秒的反应时间内，对生成的对硝基苯胺在400nm处吸光度的增加速率进行了评估。按照本文所述方法和选定方法分析的54份人血清结果相关性良好（r = 0.986）。GEMSAEC上的这种适配方法可用于检测新生儿α1-抗胰蛋白酶缺乏症。
