Hutson J C
Diabetologia. 1984 Feb;26(2):155-8. doi: 10.1007/BF00281125.
The purpose of the present study was to determine whether the secretion of androgen-binding protein and lactate by 16-day-old rat Sertoli cells is altered when these cells are cultured in simulated diabetic conditions. Incorporation of 3H-uridine into RNA by peritubular cells cultured in simulated diabetic conditions was also studied. The cells were exposed to various concentrations of glucose, beta-hydroxybutyrate, sodium bicarbonate (to alter the pH of the medium), mannitol (to influence the osmolarity of the medium), or a combination of these compounds. All of the metabolic parameters when tested alone or in combination were capable of increasing lactate secretion by Sertoli cells above control values. Basal secretion of androgen-binding protein, however, was not influenced by an individual component or when all components were tested together. FSH-stimulated levels of androgen-binding protein secretion was depressed only when the Sertoli cells were exposed to all the components simultaneously. Incorporation of uridine by peritubular cells was decreased by exposing the cells to butyrate or mannitol, while no effect was observed with glucose treatment. These results indicate that Sertoli cell and peritubular cell function can be directly altered by several specific metabolic parameters associated with diabetes.
本研究的目的是确定16日龄大鼠支持细胞在模拟糖尿病条件下培养时，其雄激素结合蛋白和乳酸的分泌是否会发生改变。同时还研究了在模拟糖尿病条件下培养的睾丸间质细胞将3H-尿苷掺入RNA的情况。将细胞暴露于不同浓度的葡萄糖、β-羟基丁酸、碳酸氢钠（以改变培养基的pH值）、甘露醇（以影响培养基的渗透压）或这些化合物的组合中。单独或组合测试时，所有代谢参数均能使支持细胞的乳酸分泌量高于对照值。然而，雄激素结合蛋白的基础分泌不受单个成分的影响，也不受所有成分一起测试时的影响。仅当支持细胞同时暴露于所有成分时，FSH刺激的雄激素结合蛋白分泌水平才会降低。将睾丸间质细胞暴露于丁酸或甘露醇中会降低其尿苷掺入量，而葡萄糖处理则未观察到影响。这些结果表明，与糖尿病相关的几个特定代谢参数可直接改变支持细胞和睾丸间质细胞的功能。
