[Regulation of the cellular action of antidiuretic hormone].

The steps of cell reactions which could modulate the effect of the antidiuretic hormone (ADH) were investigated in experiments on frog urinary bladder. Adrenaline and D2O reduced the interaction between ADH and its receptors. The urinary bladder cells released an inhibitor of ADH changing the reaction of receptors to ADH; adsorption of this inhibitor increased the water permeability after addition of ADH. Increased intracellular concentration of cellular near basolateral membranes produced the increase of water permeability whereas near the apical membranes calcium produced its decrease acting, perhaps, on microtubules. Swelling of the cells caused by ADH didn't change the reaction of these cells to ADH. Nevertheless, the cells swollen in hypotonic solution before the application ADH showed a lesser reaction to ADH. The role of cAMP phosphodiesterase, hyaluronidase, aldosterone, prostaglandins and other physiologically active substances in the action of ADH has been discussed. The data obtained suggest some possible ways and mechanisms of regulation of the cellular action of ADH.