蛋白质与高碘酸盐氧化的葡聚糖共价偶联：免疫吸附剂制备的一种新方法。
The covalent coupling of proteins to periodate-oxidized sephadex: a new approach to immunoadsorbent preparation.
作者信息
Wilson M B, Nakane P K
出版信息
J Immunol Methods. 1976;12(1-2):171-81. doi: 10.1016/0022-1759(76)90107-1.
PMID:62811
Abstract
Proteins were covalently coupled in good yield to periodate-oxidized Sephadex. The reaction was studied in detail with respect to Sephadex type, sodium m-periodate concentration, protein (albumin or immunoglobulin) concentration, time course, temperature dependence, and pH optimum. The complexes were stable and were used successfully for the immunoadsorption of sheep anti-rabbit immunoglobulin antibodies.
摘要
蛋白质以良好的产率共价偶联到高碘酸盐氧化的葡聚糖凝胶上。针对葡聚糖凝胶类型、间高碘酸钠浓度、蛋白质（白蛋白或免疫球蛋白）浓度、时间进程、温度依赖性和最适pH对该反应进行了详细研究。这些复合物很稳定，并成功用于羊抗兔免疫球蛋白抗体的免疫吸附。
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