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利用单克隆抗体对单纯疱疹病毒感染细胞内存在的DNA结合蛋白进行表征和纯化。

The characterization and purification of DNA binding proteins present within herpes simplex virus infected cells using monoclonal antibodies.

作者信息

la Thangue N B, Chan W L

出版信息

Arch Virol. 1984;79(1-2):13-33. doi: 10.1007/BF01314300.

DOI:10.1007/BF01314300
PMID:6320770
Abstract

Hybridomas secreting monoclonal antibodies against herpes simplex virus (HSV) DNA binding proteins (DBP) have been produced. Five HSV DBP have been characterized according to molecular weight, affinity for DNA, kinetic class and localization within the infected cell. By preparing an immunoadsorbent column from antibody TI8, its specific DBP was purified to apparent homogeneity. The purified DBP retained the ability to bind to DNA.

摘要

已制备出分泌抗单纯疱疹病毒（HSV）DNA结合蛋白（DBP）单克隆抗体的杂交瘤。已根据分子量、对DNA的亲和力、动力学类别以及在受感染细胞内的定位对5种HSV DBP进行了表征。通过用抗体TI8制备免疫吸附柱，其特异性DBP被纯化至表观均一性。纯化后的DBP保留了与DNA结合的能力。

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The characterization and purification of DNA binding proteins present within herpes simplex virus infected cells using monoclonal antibodies.利用单克隆抗体对单纯疱疹病毒感染细胞内存在的DNA结合蛋白进行表征和纯化。
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引用本文的文献

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本文引用的文献

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lambda Repressor and cro--components of an efficient molecular switch.λ阻遏蛋白与cro——一种高效分子开关的组成部分。
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Monoclonal antibodies to three non-glycosylated antigens of herpes simplex virus type 2.针对单纯疱疹病毒2型三种非糖基化抗原的单克隆抗体。
J Gen Virol. 1982 Dec;63(2):297-305. doi: 10.1099/0022-1317-63-2-297.
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DNA-binding properties of a herpes simplex virus immediate early protein.单纯疱疹病毒立即早期蛋白的DNA结合特性
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Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4.针对1型单纯疱疹病毒蛋白的单克隆抗体，包括即刻早期蛋白ICP 4。
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