评估一种用于从接种的牡蛎组织匀浆中提取脊髓灰质炎病毒的错流过滤技术。
Evaluation of a cross-flow filtration technique for extraction of polioviruses from inoculated oyster tissue homogenates.
作者信息
Richards G P, Goldmintz D
出版信息
J Virol Methods. 1982 Apr;4(3):147-53. doi: 10.1016/0166-0934(82)90043-x.
Pleated membrane filtration capsules, utilizing cross-flow and liquid membrane technology, were evaluated for use in poliovirus extraction from inoculated oyster tissue homogenates. The filtration technique is fast and less complex than other extraction procedures. Capsules filtered an average of 450.3 ml of a one liter homogenate within 20 min. Filtrates were clear and contained a mean virus recovery of 46.1% in non-dialyzed samples that were concentrated by ultrafiltration. Cytotoxicity was not observed in cell culture monolayers inoculated with non-dialyzed, water-dialyzed, or buffer-dialyzed concentrates. Dialysis and concentration procedures did not significantly reduce the number of viruses in the filtrates.
利用错流和液膜技术的褶式膜过滤胶囊，被评估用于从接种的牡蛎组织匀浆中提取脊髓灰质炎病毒。该过滤技术比其他提取程序更快且更简单。胶囊在20分钟内平均过滤了一升匀浆中的450.3毫升。滤液清澈，在通过超滤浓缩的未透析样品中，病毒平均回收率为46.1%。在用未透析、水透析或缓冲液透析的浓缩物接种的细胞培养单层中未观察到细胞毒性。透析和浓缩程序并未显著减少滤液中的病毒数量。
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