从牡蛎中回收自然积累的脊髓灰质炎病毒的改良程序。
Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
作者信息
Vaughn J M, Landry E F, Vicale T J, Dahl M C
出版信息
Appl Environ Microbiol. 1979 Oct;38(4):594-8. doi: 10.1128/aem.38.4.594-598.1979.
Abstract
Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters.
摘要
对通过正常过滤活动积累病毒的牡蛎（太平洋牡蛎）中脊髓灰质炎病毒的回收方法进行了比较。澄清程序包括甘氨酸 - 氯化钠和聚电解质提取方法，随后采用多种酸沉淀浓缩方法。聚电解质絮凝后在pH 3.5下进行牛肉提取物补充的酸沉淀，回收率最高。发现对匀浆进行直接检测是确定“自然感染”牡蛎中初始病毒浓度的不可靠方法。
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