Effect of maleate on membrane physical state of brush border and basolateral membranes of the dog kidney.

The effects of maleate on the physical state of isolated brush border and basolateral membranes from dog kidney cortex have been studied by fluorescence polarization and ESR methods. Anisotropy of 1,6-diphenyl-1.3.5.-hexatriene and hyperfine splitting (2T parallel) of 5-doxylstearic acid in brush border and basolateral membranes were not significantly modified by the addition of 10(-2) M maleate after 60-90 min treatment. These findings further support the view that maleic acid nephropathy is not due to a direct effect of maleate on tubular membranes per se.