Gascon-Barré M
Metabolism. 1982 Jan;31(1):67-72. doi: 10.1016/0026-0495(82)90028-2.
Vitamin D [D] depleted female Sprague-Dawley rats were fed for a period of 4 wk a D deficient diet containing 36% of total calories as ethanol while control animals received an isocaloric regimen where ethanol was substituted for by dextrins. In conjunction with the ethanol feeding 92 I.U. of [14C]-vitamin D3 [(14C)-D3] were administered by intragastric gavage 3 times 1 wk for 3 2/3 wk. At the end of the experiment, [14C]-D3 and [14C]-25-hydroxyvitamin D3 [(14C)-25(OH)D3] concentrations were analyzed in plasma, liver, striated muscle and adipose tissue. Body reserves in unchanged [14C]-D3 were significantly reduced by ethanol treatment as seen by 24%, 26%, and 59% lower plasma (p less than 0.02), muscle (p less than 0.001) and adipose tissue (p less than 0.001) [14C]-D3 concentrations in ethanol-treated compared to control rats. In contrast total plasma and liver [14C]-25(OH)D3 content were increased by 30% (p less than 0.05) and 55% (p less than 0.001) respectively. This increased liver and plasma [14C]-25(OH)D3 following ethanol treatment was not accompanied by a proportional [14C]-25(OH)D3 incorporation into muscle and adipose tissue. These results suggest that during steady state conditions 25(OH)D3 production is increased during chronic ethanol administration while the body pool in unchanged D3 is significantly lowered. These results also point out that in the rat plasma 25(OH)D concentrations are not a reliable guide for the determination of vitamin D status during chronic ethanol administration.
将维生素D[D]耗尽的雌性斯普拉格-道利大鼠喂食含36%总热量乙醇的维生素D缺乏饮食，持续4周，而对照动物接受等热量饮食，用糊精替代乙醇。在喂食乙醇的同时，每周1次，共3又2/3周，通过胃内灌胃给予92国际单位的[14C]-维生素D3[(14C)-D3]，共3次。实验结束时，分析血浆、肝脏、横纹肌和脂肪组织中[14C]-D3和[14C]-25-羟基维生素D3[(14C)-25(OH)D3]的浓度。与对照大鼠相比，乙醇处理使未改变的[14C]-D3的体内储备显著降低，血浆（降低24%，p<0.02）、肌肉（降低26%，p<0.001）和脂肪组织（降低59%，p<0.001）中[14C]-D3浓度均降低。相反，血浆和肝脏中总[14C]-25(OH)D3含量分别增加了30%（p<0.05）和55%（p<0.001）。乙醇处理后肝脏和血浆中[14C]-25(OH)D3增加，但肌肉和脂肪组织中[14C]-25(OH)D3的掺入并未相应增加。这些结果表明，在稳态条件下，慢性乙醇给药期间25(OH)D3的产生增加，而未改变的D3的体内储备显著降低。这些结果还指出，在大鼠中，慢性乙醇给药期间血浆25(OH)D浓度不是确定维生素D状态的可靠指标。
