Taniguchi T, Yamaguchi S, Maeda M, Kawamura H, Horiuchi T
Natl Inst Anim Health Q (Tokyo). 1981 Summer;21(2):83-93.
Rhode Island Red laying hens were inoculated with the JPA-1 strain of egg drop syndrome-1976 virus and examined pathologically 1, 3, 5, 7, 10, 14, 21, 28 and 80 days postinoculation (p.i.). Eggs with a discolored or soft shell or no shell were laid over a period from 10 to 24 days p.i. Egg production failed transiently in some affected birds after 13 days p.i. At autopsy, the uterus revealed remarkable edema and swelling of the mucosal folds together with the deposition of whitish exudate in the cavity 14 days p.i. Microscopically, intranuclear inclusion bodies were found in epithelial cells of the uterus, isthmus and vaginal gland region 10 and 14 days p.i. There were also severe degeneration and desquamation of the epithelial cells, atrophy of the uterine glands and remarkable infiltration of heterophils, lymphocytes and plasmacytes accompanied with extensive edema. Twenty-one days after inoculation, lymphoid follicles were formed in the mucosal folds of some part of the oviduct. Electron microscopic examination revealed various amounts of virus particles 68 approximately 80 nm in diameter mainly in the nuclei of the epithelial cells on the mucosa and in the exudate, and also in the cytoplasm of macrophages in the uterus.
将罗德岛红蛋鸡接种1976年蛋下降综合征病毒的JPA - 1株，并在接种后1、3、5、7、10、14、21、28和80天进行病理检查。在接种后10至24天内产出了变色、软壳或无壳的蛋。接种后13天，一些受影响的鸡的产蛋量暂时下降。尸检时，接种后14天，子宫显示出明显的水肿和黏膜皱襞肿胀，腔内有白色渗出物沉积。显微镜检查发现，接种后10天和14天，子宫、峡部和阴道腺区域的上皮细胞内有核内包涵体。上皮细胞还出现严重变性和脱落，子宫腺萎缩，嗜异性粒细胞、淋巴细胞和浆细胞显著浸润，并伴有广泛水肿。接种后21天，输卵管某些部位的黏膜皱襞中形成了淋巴滤泡。电子显微镜检查发现，直径约80纳米的各种数量的病毒颗粒主要存在于黏膜上皮细胞的细胞核和渗出物中，也存在于子宫巨噬细胞的细胞质中。
