从黏粒文库中分离和鉴定人胶原蛋白α1(I)样基因
Isolation and characterization of a human collagen alpha 1(I)-like gene from a cosmid library.
作者信息
Weiss E H, Cheah K S, Grosveld F G, Dahl H H, Solomon E, Flavell R A
出版信息
Nucleic Acids Res. 1982 Mar 25;10(6):1981-94. doi: 10.1093/nar/10.6.1981.
Abstract
We have isolated a human collagen alpha 1(I)-like gene from a cosmid library. The clone which contains 37kb of human DNA has been shown to contain this gene by DNA sequencing, hybrid arrest and hybrid selection assays and Northern blot hybridizations. The collagen gene sequence extends through most of the cloned DNA and must, therefore, be at least 35kb in length.
摘要
我们从一个黏粒文库中分离出了一个人胶原蛋白α1(I)样基因。通过DNA测序、杂交阻断和杂交筛选试验以及Northern印迹杂交，已证明包含37kb人类DNA的克隆含有该基因。胶原蛋白基因序列延伸穿过大部分克隆的DNA，因此其长度必定至少为35kb。
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