Yamada Y, Mudryj M, Sullivan M, de Crombrugghe B
J Biol Chem. 1983 Mar 10;258(5):2758-61.
We have isolated a cloned segment of the chick alpha 1 type III collagen gene from a genomic DNA library. This DNA clone was obtained by cross-hybridization with a cDNA clone containing sequences coding for a segment of the carboxy propeptide of chick alpha 1 type I collagen. This genomic clone was identified as coding for alpha 1 type III collagen by DNA sequence analysis of two segments of the gene and by its ability to hybridize to crop mRNA but not to calvaria mRNA in agreement with the presence of alpha 1 type III collagen in crop but not in calvaria. The deduced amino acid sequences reveal characteristic features for alpha 1 type III collagen and are in good agreement with the known protein sequences of calf and human alpha 1 type III collagen. The size of alpha 1 type III mRNA is about 6 kilobases, slightly larger than the mRNA for alpha 1 type I. R-loop electron microscopic analysis indicates that at least 16 exons are interspersed in a 9-kilobase DNA segment that contains the third distal part of the alpha 1 type III collagen gene.
我们从基因组DNA文库中分离出了鸡α1Ⅲ型胶原基因的一个克隆片段。该DNA克隆是通过与一个cDNA克隆进行交叉杂交获得的，该cDNA克隆包含编码鸡α1Ⅰ型胶原羧基前肽一段序列。通过对该基因的两个片段进行DNA序列分析，以及它与嗉囊mRNA杂交而不与颅骨mRNA杂交的能力（这与嗉囊中存在α1Ⅲ型胶原而颅骨中不存在相一致），确定该基因组克隆编码α1Ⅲ型胶原。推导的氨基酸序列揭示了α1Ⅲ型胶原的特征，并且与小牛和人α1Ⅲ型胶原的已知蛋白质序列高度一致。α1Ⅲ型mRNA的大小约为6千碱基，略大于α1Ⅰ型mRNA。R环电子显微镜分析表明，至少16个外显子散布在一个9千碱基的DNA片段中，该片段包含α1Ⅲ型胶原基因的第三个远端部分。
