Chia W, Scott M R, Rigby P W
Nucleic Acids Res. 1982 Apr 24;10(8):2503-20. doi: 10.1093/nar/10.8.2503.
We have constructed and characterised a series of approved, disabled cosmid vectors which we call Homer cosmids and have examined the optimal conditions for the construction of libraries of eukaryotic DNA segments using these vectors. Analysis of these libraries shows that most of the sequences we have tested for are present at the expected frequency and that the libraries can be stably propagated. We have also directly tested the stability of cosmid clones carrying tandemly repeated inserts. This work shows that it should be possible to clone most eukaryotic genes using cosmid vectors and that such cloning systems have considerable advantages over those more commonly used.
我们构建并鉴定了一系列经过改造的黏粒载体，我们将其称为霍默黏粒，并研究了使用这些载体构建真核DNA片段文库的最佳条件。对这些文库的分析表明，我们所检测的大多数序列都以预期的频率存在，并且这些文库能够稳定地扩增。我们还直接测试了携带串联重复插入片段的黏粒克隆的稳定性。这项工作表明，使用黏粒载体克隆大多数真核基因应该是可行的，并且这种克隆系统比那些更常用的系统具有相当大的优势。
