温度、pH值和通气量对粘质沙雷氏菌核酸酶产量影响的两水平析因筛选
Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease.
作者信息
Jepsen P K, Riise E, Biedermann K, Kristensen P C, Emborg C
机构信息
Department of Biotechnology, Technical University of Denmark, Lyngby.
出版信息
Appl Environ Microbiol. 1987 Oct;53(10):2593-6. doi: 10.1128/aem.53.10.2593-2596.1987.
Abstract
Two high-nuclease-yielding mutants of Serratia marcescens, derived by chemical mutagenesis (W280, W355), and two strains with the pBR322 plasmid 403-SD2, carrying a nuclease gene and a chloramphenicol resistance gene [Escherichia coli CSH50(403-SD2) and S. marcescens CH30(403-SD2)] were investigated for nuclease production in a factorial shake flask experiment, with temperature (30 and 37 degrees C), pH (with or without CaCO3 tablets), and aeration (with or without baffles) as variable conditions. Yields varied 10-fold depending on the conditions investigated.
摘要
通过化学诱变获得的两株高产核酸酶的粘质沙雷氏菌突变体（W280、W355），以及两株携带核酸酶基因和氯霉素抗性基因的pBR322质粒403-SD2菌株[大肠杆菌CSH50(403-SD2)和粘质沙雷氏菌CH30(403-SD2)]，在析因摇瓶实验中研究了核酸酶的产生情况，实验将温度（30和37摄氏度）、pH值（添加或不添加碳酸钙片）和通气情况（有或没有挡板）作为可变条件。根据所研究的条件不同，产量变化了10倍。
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2
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3
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J Biochem. 1983 May;93(5):1287-95. doi: 10.1093/oxfordjournals.jbchem.a134262.
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Nucleic Acids Res. 1982 Apr 24;10(8):2503-20. doi: 10.1093/nar/10.8.2503.
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