Harbers K, Schnieke A, Stuhlmann H, Jaenisch R
Nucleic Acids Res. 1982 Apr 24;10(8):2521-37. doi: 10.1093/nar/10.8.2521.
The Mov-2 and Mov-10 substrains of mice, each carrying Moloney leukemia virus (= M-MuLV) in their germ line at the Mov-2 and Mov-10 locus, respectively, do occasionally at a later age (Mov-2) or not at all (Mov-10) activate infectious virus. The M-MuLV proviruses with flanking mouse sequences corresponding to the Mov-2 and Mov-10 locus, respectively, were molecularly cloned. Restriction enzyme analysis revealed no major deletions or insertions in the proviral genomes of the Mov-2 and Mov-10 locus. Both cloned DNAs induced XC plaques in a transfection assay. The specific infectivity, however, was very low and 3T3 cells transfected with the Mov-2 or Mov-10 clone did not produce infectious virus. Removing part of the 5' cellular sequences from the Mov-10 clone did not increase the infectivity. The results suggest that the M-MuLV integrated at the Mov-2 and Mov-10 locus carry a mutation which prevents synthesis of infectious virus but permits XC plaque induction by partial genome expression or synthesis of non-infectious particles.
Mov-2和Mov-10品系的小鼠，分别在其生殖系的Mov-2和Mov-10位点携带莫洛尼白血病病毒（=M-MuLV），Mov-2品系的小鼠在较晚年龄偶尔会激活感染性病毒，而Mov-10品系的小鼠则根本不会激活。分别与Mov-2和Mov-10位点对应的带有侧翼小鼠序列的M-MuLV前病毒被进行了分子克隆。限制性酶切分析显示，Mov-2和Mov-10位点的前病毒基因组中没有重大缺失或插入。在转染试验中，两种克隆的DNA都能诱导产生XC噬斑。然而，其特异性感染性非常低，用Mov-2或Mov-10克隆转染的3T3细胞不会产生感染性病毒。从Mov-10克隆中去除部分5'细胞序列并不会增加其感染性。结果表明，整合在Mov-2和Mov-10位点的M-MuLV携带一种突变，该突变阻止感染性病毒的合成，但允许通过部分基因组表达或非感染性颗粒的合成诱导XC噬斑。
