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转基因Mov小鼠中的逆转录病毒整合位点常常定位在转录DNA区域附近。

Retroviral integration sites in transgenic Mov mice frequently map in the vicinity of transcribed DNA regions.

作者信息

Mooslehner K, Karls U, Harbers K

机构信息

Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.

出版信息

J Virol. 1990 Jun;64(6):3056-8. doi: 10.1128/JVI.64.6.3056-3058.1990.

DOI:10.1128/JVI.64.6.3056-3058.1990
PMID:2335826
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249491/
Abstract

Transcription of cellular sequences flanking proviral insertion sites was studied in several Mov mouse strains, each of which carried one copy of the Moloney murine leukemia virus in its germ line. In three out of five randomly chosen Mov strains, the provirus had integrated in the vicinity of DNA regions transcribed in the embryonal stem cell line CCE and the embryonal carcinoma cell line F9. Assuming that CCE and F9 cells are developmentally equivalent to the early embryonic cells that were infected to establish the Mov strains, our results suggest that retroviruses integrate preferentially into actively transcribed DNA regions.

摘要

在几种Mov小鼠品系中研究了原病毒插入位点侧翼的细胞序列转录情况，每个品系的种系中都携带一份莫洛尼氏鼠白血病病毒。在随机选择的5个Mov品系中，有3个品系的原病毒整合在了胚胎干细胞系CCE和胚胎癌细胞系F9中转录的DNA区域附近。假设CCE和F9细胞在发育上等同于感染后建立Mov品系的早期胚胎细胞，我们的结果表明逆转录病毒优先整合到活跃转录的DNA区域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2897/249491/2c31599b6d55/jvirol00061-0618-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2897/249491/5726f000652a/jvirol00061-0617-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2897/249491/2c31599b6d55/jvirol00061-0618-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2897/249491/5726f000652a/jvirol00061-0617-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2897/249491/2c31599b6d55/jvirol00061-0618-a.jpg

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