箭毒蛙毒素可改变经内部灌注的神经母细胞瘤细胞中钠通道的门控动力学。
Batrachotoxin modifies the gating kinetics of sodium channels in internally perfused neuroblastoma cells.
作者信息
Huang L Y, Moran N, Ehrenstein G
出版信息
Proc Natl Acad Sci U S A. 1982 Mar;79(6):2082-5. doi: 10.1073/pnas.79.6.2082.
Abstract
We have studied the effects of batrachotoxin (BTX) on sodium channels in hybrid mouse neuroblastoma cells NG108-15 by using the suction pipet voltage clamp method. BTX-modified sodium channels activate with first-order kinetics and, over most of the potential range, activate more slowly than normal sodium channels. The peak conductance-voltage curve and the time constant of activation-versus-voltage curve for BTX-modified sodium channels are shifted about 50 mV in the hyperpolarizing direction compared to the corresponding curves for normal sodium channels. There is no change in the slope of the conductance-voltage curve. These results suggest that BTX slows down one of the steps leading to channel opening, which consequently becomes rate-limiting. In addition, BTX eliminates both fast and slow inactivation.
摘要
我们运用吸液管电压钳法研究了蛙毒素（BTX）对杂交小鼠神经母细胞瘤细胞NG108-15中钠通道的影响。BTX修饰的钠通道以一级动力学激活，并且在大部分电位范围内，其激活速度比正常钠通道慢。与正常钠通道的相应曲线相比，BTX修饰的钠通道的峰值电导-电压曲线和激活时间常数-电压曲线在超极化方向上偏移了约50 mV。电导-电压曲线的斜率没有变化。这些结果表明，BTX减缓了导致通道开放的步骤之一，因此该步骤成为限速步骤。此外，BTX消除了快速和慢速失活。
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本文引用的文献
1
The dual effect of membrane potential on sodium conductance in the giant axon of Loligo.枪乌贼巨大轴突中膜电位对钠电导的双重作用。
J Physiol. 1952 Apr;116(4):497-506. doi: 10.1113/jphysiol.1952.sp004719.
2
TETRODOTOXIN BLOCKAGE OF SODIUM CONDUCTANCE INCREASE IN LOBSTER GIANT AXONS.河豚毒素对龙虾巨轴突中钠电导增加的阻断作用
J Gen Physiol. 1964 May;47(5):965-74. doi: 10.1085/jgp.47.5.965.
3
Quantitative description of sodium currents in myelinated nerve fibres of Xenopus laevis.非洲爪蟾有髓神经纤维中钠电流的定量描述。
J Physiol. 1960 Jun;151(3):491-501. doi: 10.1113/jphysiol.1960.sp006455.
4
Sodium currents in the myelinated nerve fibre of Xenopus laevis investigated with the voltage clamp technique.用电压钳技术研究非洲爪蟾有髓神经纤维中的钠电流。
J Physiol. 1959 Oct;148(1):188-200. doi: 10.1113/jphysiol.1959.sp006281.
5
A quantitative description of membrane current and its application to conduction and excitation in nerve.膜电流的定量描述及其在神经传导和兴奋中的应用。
J Physiol. 1952 Aug;117(4):500-44. doi: 10.1113/jphysiol.1952.sp004764.
6
The suction pipette method for internal perfusion and voltage clamp of small excitable cells.用于小的可兴奋细胞内部灌注和电压钳制的吸引移液管方法。
J Neurosci Methods. 1980 Feb;2(1):51-78. doi: 10.1016/0165-0270(80)90045-x.
7
Covalent labeling of protein components of the sodium channel with a photoactivable derivative of scorpion toxin.用蝎毒素的光活化衍生物对钠通道的蛋白质成分进行共价标记。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):639-43. doi: 10.1073/pnas.77.1.639.
8
9
The influence of external potassium on the inactivation of sodium currents in the giant axon of the squid, Loligo pealei.外部钾离子对乌贼（莱氏拟乌贼）巨大轴突中钠电流失活的影响。
J Gen Physiol. 1969 Jun;53(6):685-703. doi: 10.1085/jgp.53.6.685.
10
Effects of batrachotoxin on membrane potential and conductance of squid giant axons.蟾毒素对鱿鱼巨大轴突膜电位和电导率的影响。
J Gen Physiol. 1971 Jul;58(1):54-70. doi: 10.1085/jgp.58.1.54.
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