前列腺素及花生四烯酸代谢的其他产物对麻疹病毒在非洲绿猴肾细胞中复制的差异作用。
Differential effect of prostaglandins and other products of arachidonic acid metabolism on measles virus replication in Vero cells.
作者信息
Dore-Duffy P
出版信息
Prostaglandins Leukot Med. 1982 Jan;8(1):73-82. doi: 10.1016/0262-1746(82)90129-9.
The influence of cyclic adenosine 3', 5'-monophosphate (cAMP) and prostaglandin (PG), E1, E2, F2 alpha, A2 and thromboxane B2 (TxB2) and measles virus infection was investigated. Addition of PGE1, E2 and cAMP (10(-3)-10(-)8M) inhibited measles virus replication in Vero cells. TxB2 and PGA2 enhanced replication. Cytotoxic effects were not observed. Inhibition of infectious titers (98%) was most pronounced when agents were present throughout replication. Treated cells exhibited hemadsorbing antigen, but the size and number of cytoplasmic inclusions was decreased by cAMP. Pre-treatment of cells with PGE1 or presence of PGE1 during virus adsorption enhanced infectious titers (30%). Results suggest that cAMP and PGE1 inhibit measles virus production infection. That both enhancing and inhibiting effects of PGs were seen suggest that measles virus may require PG at specific times during the replicative cycle.
研究了环磷酸腺苷（cAMP）、前列腺素（PG）E1、E2、F2α、A2以及血栓素B2（TxB2）和麻疹病毒感染的影响。添加PGE1、E2和cAMP（10⁻³ - 10⁻⁸M）可抑制麻疹病毒在Vero细胞中的复制。TxB2和PGA2可增强复制。未观察到细胞毒性作用。当在整个复制过程中都存在这些因子时，对感染滴度的抑制作用（98%）最为明显。经处理的细胞表现出血细胞吸附抗原，但cAMP可使细胞质内含物的大小和数量减少。用PGE1对细胞进行预处理或在病毒吸附期间存在PGE1可提高感染滴度（30%）。结果表明，cAMP和PGE1可抑制麻疹病毒的产生和感染。PGs既有增强作用又有抑制作用，这表明麻疹病毒在复制周期的特定时间可能需要PG。
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