Merkel K H, Zimmer M
Am J Clin Pathol. 1982 May;77(5):605-7. doi: 10.1093/ajcp/77.5.605.
Ten rats were inoculated intracerebrally with Herpes simplex virus type I to study the possibility of establishing a rapid diagnosis of Herpes simplex encephalitis through examination of touch preparations of brain tissue stained by the immunoperoxidase method. All except one of the animals died within four days of the developing encephalitis. The surviving animal was killed on the fourth day. Touch preparations were shortly fixed in 4% buffered formalin, cold acetone, or alcohol. The indirect immunoperoxidase method was used to identify viral antigen. The authors found that formalin serves as a good fixative and sensitivity as well as specificity is high. The diagnosis could be made within 4 hours. The cellular morphology is retained. The reaction product is stable and background staining no problem. The results of this study imply to use this method also for rapid diagnosis of brain biopsy tissue in human infections.
用I型单纯疱疹病毒对10只大鼠进行脑内接种，以研究通过检查经免疫过氧化物酶法染色的脑组织触片来快速诊断单纯疱疹性脑炎的可能性。除1只动物外，所有动物在脑炎发生后的4天内死亡。存活的动物在第4天被处死。触片立即用4%缓冲甲醛、冷丙酮或酒精固定。采用间接免疫过氧化物酶法鉴定病毒抗原。作者发现，甲醛是一种良好的固定剂，灵敏度和特异性都很高。诊断可在4小时内做出。细胞形态得以保留。反应产物稳定，背景染色无问题。本研究结果表明，该方法也可用于人类感染时脑活检组织的快速诊断。
