Tomlinson A H, Chinn I J, MacCallum F O
J Clin Pathol. 1974 Jun;27(6):495-9. doi: 10.1136/jcp.27.6.495.
Direct immunofluorescence staining for herpes simplex virus was applied to cryostat sections of 43 specimens removed at brain biopsy. Herpes antigen was found in 10 specimens and virus was isolated from them. Antigen was found in one specimen from which virus was not isolated. Two specimens from which virus was isolated gave equivocal fluorescence. Thirty specimens gave no fluorescence or live virus. Immunofluorescence provided a diagnosis in three hr compared with 24-42 hr for virus isolation. Indirect immunofluorescence staining was applied to sections of brain removed at necropsy and fixed in formalin. Herpes antigen was found in sections of six of the 12 brains examined.
对43例脑活检取出的标本进行低温切片，应用单纯疱疹病毒直接免疫荧光染色。在10例标本中发现疱疹抗原，并从中分离出病毒。在1例标本中发现抗原，但未分离出病毒。2例分离出病毒的标本荧光不明确。30例标本未出现荧光或活病毒。免疫荧光检测在3小时内即可做出诊断，而病毒分离则需要24 - 42小时。对尸检时取出并固定于福尔马林中的脑切片进行间接免疫荧光染色。在检查的12例脑标本中，有6例切片发现疱疹抗原。
