Cotugno M, Sansone G, Gallone U, Barone E, Rossi L, Biondi A
Boll Soc Ital Biol Sper. 1982 Mar 30;58(6):295-9.
Purification methods of vegetable extracts have been experienced during our researches on the possible presence of the aromatic polycyclic hydrocarbons (particularly 3,4 benzopyrene) in vegetables. The validity of different methods has been determined by IR spectrophotometric analysis using special apparatus (IR-DATA-STATION). Vegetables extracts have been purified efficaciously by column chromatography, saponification and special methods (Grimmer's method). The described method eliminates, nearly entirely, the polluting substances that could alternate the chemical analysis of the vegetable extracts.
在我们对蔬菜中可能存在的芳香多环烃(特别是3,4-苯并芘)的研究过程中,经历了蔬菜提取物的纯化方法。不同方法的有效性已通过使用特殊仪器(红外数据站)的红外分光光度分析来确定。蔬菜提取物已通过柱色谱法、皂化法和特殊方法(格林默法)有效地纯化。所描述的方法几乎完全消除了可能干扰蔬菜提取物化学分析的污染物质。