Harlan R E, Shivers B D, Kow L M, Pfaff D W
Brain Res. 1982 Apr 22;238(1):153-67. doi: 10.1016/0006-8993(82)90778-8.
Since some estrogenic effects on lordotic responsiveness are mediated through hypothalamic protein synthesis, we conducted experiments to determine if axoplasmic transport in the hypothalamus is necessary for the induction and maintenance of this reflex by estrogen. Colchicine infusion into the hypothalamus, but not into the dorsal thalamus, of ovariectomized rats 24 h prior to administration of subcutaneous estrogen implants delayed the induction of lordotic responsiveness, as measured by the manual (cutaneous-pressure) method, by 2 days, as compared with vehicle-infused rats. In other experiments, colchicine infusion into the hypothalamus, but not into the dorsal thalamus, of conscious, ovariectomized, estrogen-implanted rats displaying maximal lordotic responsiveness resulted in a bimodal decline in lordotic responsiveness. An initial decline occurred 20-40 min after infusion, and was associated with general behavioral agitation and hyperactivity. A subsequent decline began 4 h after infusion and lasted for several days. Vehicle infusion did not decrease lordotic responsiveness. Colchicine infusion did not alter multiunit electrical activity recorded near hypothalamically directed cannulae tips over a period of several hours. Results suggest that axoplasmic transport within and/or from the hypothalamus is necessary for the estrogenic induction and maintenance of the lordosis reflex in rats.
由于雌激素对脊柱前凸反应性的某些影响是通过下丘脑蛋白质合成介导的，我们进行了实验，以确定下丘脑的轴浆运输对于雌激素诱导和维持这种反射是否必要。在皮下植入雌激素前24小时，给去卵巢大鼠的下丘脑而非背侧丘脑注入秋水仙碱，与注入赋形剂的大鼠相比，通过手法（皮肤压力）测量，脊柱前凸反应性的诱导延迟了2天。在其他实验中，给表现出最大脊柱前凸反应性的清醒、去卵巢、植入雌激素的大鼠的下丘脑而非背侧丘脑注入秋水仙碱，导致脊柱前凸反应性出现双峰下降。最初的下降发生在注入后20 - 40分钟，与一般行为激动和多动有关。随后的下降在注入后4小时开始，持续数天。注入赋形剂并未降低脊柱前凸反应性。在数小时内，注入秋水仙碱并未改变下丘脑定向套管尖端附近记录的多单位电活动。结果表明，下丘脑内和/或来自下丘脑的轴浆运输对于大鼠雌激素诱导和维持脊柱前凸反射是必要的。
